His-Tag-Epitope Imprinted Thermoresponsive Magnetic Nanoparticles for Recognition and Separation Thyroid Peroxidase Antigens from Whole Blood Samples


Turan E.

CHEMISTRYSELECT, vol.3, no.42, pp.11963-11969, 2018 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 3 Issue: 42
  • Publication Date: 2018
  • Doi Number: 10.1002/slct.201801557
  • Journal Name: CHEMISTRYSELECT
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.11963-11969
  • Keywords: His-tag-epitope, molecular imprinted, specific recognition, separation, thyroid peroxidase, SURFACE, POLYMERS, BIOSENSORS, HYDROGELS, SITE
  • Gazi University Affiliated: Yes

Abstract

In the epitope imprinting method, a short peptide, even without a defined tertiary structure, can be used as the template to produce protein-selective polymers. In this study, His-tag-epitope imprinted magnetic nanoparticles (EMIP-MNPs) were prepared via epitope imprinting method onto the surface of the magnetic nanoparticles for specific recognition and separation of thyroid peroxidase antigens (TPO). 3-(Trimethoxysilyl) propyl methacrylate (TMSPM), copper (II) acrylate (Cu(II)Ac) and N-isopropylacrylamide (NIPAAm) were chosen as functional monomers. Ethyleneglycoldimethacrylate (EGDMA) and 2,2'-azobisisobutyronitrile (AIBN) were selected as crosslinking agent and initiator, respectively. The EMIP-MNPs were prepared within 30 min by epitope imprinting method which was shorter than the surface imprinting method. The prepared EMIP-MNPs were thermosensitive and showed selective affinity to target TPO antigens. Moreover, the EMIP-MNPs were easily and quickly recognized and separated to target antigens from the whole blood sample.