Studies of the binding interactions of dicationic styrylimidazo[1,2-a] pyridinium dyes with duplex and quadruplex DNA


SEFEROĞLU Z., Mahmoud M. M. A. , Ihmels H.

DYES AND PIGMENTS, vol.125, pp.241-248, 2016 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 125
  • Publication Date: 2016
  • Doi Number: 10.1016/j.dyepig.2015.10.008
  • Journal Name: DYES AND PIGMENTS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.241-248
  • Keywords: Double cationic styryl dyes, Imidazo[1,2-a]pyridines, Nucleic acids, ct DNA, Quadruplex DNA, DNA-ligand interactions, PHOTOPHYSICAL PROPERTIES, NUCLEIC-ACIDS, FLUORESCENT, DERIVATIVES, LIGANDS, PRINCIPLES, SERIES
  • Gazi University Affiliated: Yes

Abstract

The dicationic styrylimidazo[1,2-a]pyridinium dyes were synthesized and characterized using H-1 NMR, C-13 NMR, mass spectral techniques and elemental analysis. In addition, their photophysical and DNA-binding properties were investigated. The dyes absorb in the UV region with maxima at 342-358 nm, and the emission spectra of these derivatives show a maximum at 409-475 nm with moderate Stokes shift. It was shown with spectrophotometric, spectrofluorimetric and viscometric titrations as well as with Circular dichroism spectroscopic analysis that the dicationic styrylimidazo[1,2-a]pyridinium dyes associate as groove binders with double-stranded DNA with binding constants in the range of 10(5)-10(6) M-1. Preliminary experiments revealed that these ligands also bind with reasonable affinity to telomeric quadruplex DNA. (C) 2015 Elsevier Ltd. All rights reserved.