Evaluation of heat shock protein 70 and toll-like receptor 4 expression in gingival crevicular fluid in response to orthodontic forces

Bozkaya E., Canigür Bavbek N., Işler S. Ç., Uraz A., Ilikci Sagkan R., Uzunok B., ...More

CLINICAL ORAL INVESTIGATIONS, vol.25, no.11, pp.6455-6464, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 25 Issue: 11
  • Publication Date: 2021
  • Doi Number: 10.1007/s00784-021-04014-3
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, EMBASE, MEDLINE
  • Page Numbers: pp.6455-6464
  • Keywords: Heat shock protein 70, Toll-like receptor 4, Interleukin-1beta, Gingival crevicular fluid, Orthodontic tooth movement, HEAT-SHOCK PROTEINS, NECROSIS-FACTOR-ALPHA, TOOTH MOVEMENT, INTERLEUKIN-1-BETA LEVELS, CYTOKINES, RECOGNITION, BIOLOGY, RANKL, HSP70
  • Gazi University Affiliated: Yes


Objectives The aim of this study was to assess the concentrations of heat shock protein 70 (HSP70) and toll-like receptor 4 (TLR4) during orthodontic tooth movement and to compare their levels with interleukin-1 beta (IL-1 beta), a well-known proinflammatory biomarker. Materials and methods This study consisted of 20 patients (8 males, 12 females; mean age 14.75 +/- 2.34 years) who needed maxillary premolar extraction and segmental canine distalization. Concentrations of HSP70, TLR4, and IL-1 beta were examined before extraction (T1), at the 1st (T2), 4th (T3), 7th (T4), 14th (T5), and 30th (T6) days of canine retraction by enzyme-linked immunosorbent assay analysis of gingival crevicular fluid samples. Statistical analyses were performed with repeated measure ANOVA and Spearman's rank correlation coefficient analysis (p < 0.05). Results HSP70 increased gradually from T1 to T6 and showed significant differences between T1-T6 and T2-T6 (T1:3.28 +/- 0.92 ng/ml; T2:3.72 +/- 0.66 ng/ml; T6:9.35 +/- 2.45 ng/ml). The lowest TLR4 concentration was at T1, peaked at T3 and remained constant afterwards with significant differences between T1-T3, T1-T4, and T1-T6 (T1:0.71 +/- 0.02 pg/ml; T3:1.04 +/- 0.11 pg/ml; T4:0.95 +/- 0.06 pg/ml; T6:1.00 +/- 0.07 pg/ml). IL-1 beta increased from T1 to T6 with significant differences between T1-T4, T1-T5, and T1-T6 (T1:55.71 +/- 5.48 pg/ml; T4:100.11 +/- 16.92 pg/ml; T5:103.71 +/- 23.19 pg/ml; T6:125.12 +/- 22.04 pg/ml). The increase in HSP70 and TLR4 from T2-T3 showed a significant correlation (r = 0.598; p = 0.005). Conclusions The increased levels of HSP70, TLR4, and IL-1 beta show the contribution of these mediators to the inflammatory response from the early stages of orthodontic tooth movement.