Capillary electrophoretic determination of citalopram in pharmaceutical tablets and serum

Satana E., UYSAL Ü. D., Goger N., Tuncel M.

CHROMATOGRAPHIA, vol.64, pp.317-320, 2006 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 64
  • Publication Date: 2006
  • Doi Number: 10.1365/s10337-006-0007-7
  • Journal Name: CHROMATOGRAPHIA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.317-320
  • Keywords: capillary electrophoresis, citalopram in tablets, serum analysis, method development, PERFORMANCE LIQUID-CHROMATOGRAPHY, SEROTONIN REUPTAKE INHIBITORS, SOLID-PHASE EXTRACTION, ACTIVE METABOLITES, ANTIDEPRESSANTS, PLASMA, DRUG, DESMETHYLCITALOPRAM, PHARMACOKINETICS, MICROEXTRACTION
  • Gazi University Affiliated: Yes

Abstract

Determination of citalopram by capillary electrophoresis is described. Compounds were separated at 28 kV in 75 mu m i.d. fused silica capillary tubing (total length 85 cm, effective length 65 cm) with 10 mM borate buffer, pH 8.5, containing 10% (v/v) methanol as running buffer. Citalopram and propylparaben (IS) appeared at 3.5 and 5.5 min, respectively. Repeatable linear results were obtained. The limits of detection and quantification were 5.73 x 10(-6) and 1.72 x 10(-5) M, respectively. When citalopram was determined in a pharmaceutical tablet by capillary electrophoresis and by a UV-spectrophotometric method differences between the results were not significant. The citalopram content of tablets was 100.8 +/- 2.95% of the label claim. The amount found in serum was 26.7 +/- 0.1% of the free drug, indicating that 73.3% of the drug was bound to protein.