Enhanced expression of the local haematopoietic bone marrow renin-angiotensin system in polycythemia Rubra vera

Aksu S., BEYAZIT Y., Hazneidaroglu I., KEKİLLİ M., Canpinar H., Misirlioglu M., ...More

JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, vol.33, no.6, pp.661-667, 2005 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 6
  • Publication Date: 2005
  • Doi Number: 10.1177/147323000503300608
  • Journal Name: JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.661-667
  • Keywords: angiotensin-converting enzyme, erythropoiesis, flow cytometry, polycythemia Rubra vera, real-time polymerase chain reaction, renin-angiotensin system, I-CONVERTING-ENZYME, ACE-INHIBITORS, STROMAL CELLS, HYPOTHESIS
  • Gazi University Affiliated: No

Abstract

Local bone marrow (BM) renin-angiotensin system (RAS) affects physiological and pathological haematopoiesis, including erythropoiesis. In this study, quantitative expression of the messenger RNAs of the major RAS components - angiotensin-converting enzyme (CD143), renin and angiotensinogen - were measured in BM samples by quantitative real-time polymerase chain reaction, to evaluate the activity of local BM RAS in polycythemia rubra vera (PV) in comparison with normal erythropoiesis. The presence of CD143 was also investigated in the same BM samples by flow cytometry. Increased local synthesis of the major RAS components has been identified by demonstrating corresponding mRNAs in the BM of the patients with PV. Our findings indicate up-regulation of local BM RAS, together with down-regulation of the cell surface angiotensin-converting enzyme receptors, in the autonomous neoplastic clonal erythropoiesis of PV.