In this work, ten suspensions of graphene, graphene oxide and MoS2 were prepared by sonically assisted exfoliation. Electrode modification was achieved by dropping-drying method onto glassy carbon (GCE) electrode. Electrocatalytic activity against ascorbic acid (AA) was determined by the use of configured different modified electrodes. Measurements were achieved by differential pulse voltammetry (DPV). Electrode modification provided 2.8-fold increase at the precision. The results showed that catalytically active MoS2 modified electrode have 7.26 mu A mM-1of precision, 27 mu M -1100 mu M of linear working range and 8.1 mu M of limit of detection at AA determination. In the real sample analysis, AA determination was achieved with 5.05 % relative error. The thickness of layer and surface characterization of modified electrode were characterized by atomic force microscopy (AFM) and scanning electron microscopy (SEM), respectively.