JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI, vol.13, no.2, pp.95-100, 2007 (SCI-Expanded)
Article / Article
JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI
Science Citation Index Expanded (SCI-EXPANDED), TR DİZİN (ULAKBİM)
Helianthus annuus L, sunflower, immature embryo, immature cotyledons, in vitro, PLANT-REGENERATION, SOMATIC EMBRYOGENESIS, HIGH-FREQUENCY, MATURE COTYLEDONS, CULTURE, ORGANOGENESIS, INDUCTION, CALLUS, TRANSFORMATION, PROTOPLASTS
Gazi University Affiliated:
Immature embryo and cotyledons of 10 lines of sunflower (Helianthus annuus L) were cultured on Murashige and Skoog (MS) medium supplemented with 20 mg/l sucrose and different concentrations of Kinetin and alpha Naphthalene acetic acid (NAA). Development of precocious roots was observed after one week of culture, which were removed aseptically to induce callus and shoot regeneration. Induction of healthy shoots was observed on cotyledon and embryo explants on MS medium containing any concentration of kinetin and and NAA. This is an important development in the tissue culture research of sunflower.