THE ANTIMICROBIAL EFFECT OF R-LIMONENE NANOEMULSION ON ENTEROCOCCUS FAECALIS

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Yılmaz A., Şeker İ. D., Akca G., Takka S., Alaçam T.

3rd International Gazi Pharma Symposium Series (GPSS), Ankara, Türkiye, 8 - 10 Eylül 2021, ss.142

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Ankara
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.142
  • Gazi Üniversitesi Adresli: Evet

Özet

R-limonene is a terpene derivative, which has antimicrobial activity. The aim of this study is to evaluate the antibacterial efficacy of R-limonene nanoemulsions against Enterococcus faecalis, which is highly resistant to repetitive applications of calcium hydroxide used in the chemomechanical preparation of root canal treatment. Oil/water R-limonene nanoemulsions were prepared by phase inversion method. Formulation factors are listed in the table below (Table 1). The particle size (PS), polydispersity index (PDI) and zeta potential (ZP) values were evaluated and were given in the table below (Table 2). It was observed that the ZP values decreased as the Tween 80 ratio increased. Therefore, F3, F4 and F6 formulations were eliminated. F1, F2 and F5 formulations were subjected to a oneweek stability test at 4, 25 and 40 °C and it was observed that the ZP of the F1 formulation decreased at all temperatures. There were not significant changes in the F2 and F5 formulations. The MIC values of F2 and F5 formulations were determined compared to pure Rlimonene on Enterococcus faecalis. The MIC values of pure R limonene, F2 and F5 were 210.3 mg/mL, 49.80 mg/mL and 48.82 mg/mL, respectively. These results show that emulsion formulations of R-limonene are approximately 4.5 times more effective than pure R-limonene.