Anchusa limbata Boiss. & Heldr., a very rare species endemic to SW Anatolia, has been rediscovered after more than 150 years since its original collection by T. von Heldreich. Based on the new collection, a detailed morphological description and an original iconography are given to allow a better knowledge of this plant. Micromorphological and karyological aspects are also illustrated for the first time. Geographical and ecological data are reported together with an evaluation of the conservation status of this species. The taxonomic position and possible systematic relationships within the genus Anchusa are briefly discussed in the light of the new data.