Application of local anesthetics (LA) into the surgical site for postoperative analgesia is a common procedure, either by means of continuous infusion or single dose. However, LA's are toxic to neuronal cells, Schwann cells, fibroblasts, keratinocytes, leukocytes and to chondrocytes. So, we studied the apoptotic effects of bupivacaine and levobupivacaine in human fibroblasts and lymphocytes at 7.69 mu M, 76.9 mu M and 3845 mu M concentrations for 6h, 24h and 48h. Our findings revealed that, at 6 hours of the both treatments, fibroblasts showed significant difference between untretaed and the groups at 7.69 mu M, 76.9 mu M and 384.5 mu M doses, whereas only at 3845 mu M dose apoptosis was significantly different regarding lymphocytes (p<0.05). As a conclusion, bupivacaine and levobupivacaine trigger apoptosis with either increased time of exposure or increased concentration in both fibroblasts and lymphocytes. Apoptotic effects of these LA can be minimized by using them at clinically proven sufficient doses, but not at higher concentrations as traditional practice advices.