Akademik Veri Yönetim Sistemi
IBRO 11TH WORLD CONGRES OF NEUROESCIENCE, Granada, İspanya, 9 - 13 Eylül 2023, cilt.15, ss.362
THE EFFECTS OF BILATERAL TRANSAURICULAR VAGUS NERVE STIMULATION ON LPS-INDUCED ACUTE INFLAMMATION AND PAIN BEHAVIOR (B26)
Topic
AS07 Aging and Neurodegenerative Disorders
Authors
1 2 , 3, 4 5 6 7 8 7 9 Atalar K. , Alim E. , Çalıkusu A. , Topa E. , Gök Dagıdır H. , Bahcelioglu M. , Bukan N. , Bolay H.
Affiliations
1 - Department of Anatomy, Faculty of Medicine, and Neuroscience and Neurotechnology Center of Excellence NÖROM, Gazi University, Ankara, Turkey, Gazi University, Ankara, Turkey, 2 - Neuroscience, Biochemistry, Gazi University, Ankara, Turkey, 3 - Anatomy, Gazi University, Ankara, Turkey, 4 - Department of Anatomy, Faculty of Medicine, and Neurotechnology Center of Excellence NÖROM, Gazi University, Ankara, Turkey, 5 - Institute of Health Sciences Department of Neuroscience, and NÖROM, Gazi University, Ankara, Turkey, 6 - Gazi University, Neuropsychiatry Education Research and Application Center, Ankara, Türkiye, Gazi University, Ankara, Turkey, 7 - Medical Biochemistry, Gazi University, Ankara, Turkey, 8 - Department of Anatomy, Faculty of Medicine, and Neuroscience and Neurotechnology Center of Excellence NÖROM, Gazi University, Ankara, Turkey, 9 - Faculty of Medicine, Departments Neurology and Algology, and NÖROM, Gazi University, Ankara, Turkey
Abstract Body
Autonomic dysfunction might be addressed by cholinergic anti-inflammatory therapy. The vagus nerve, the major nerve of the parasympathetic nervous system, may modulate how the immune system reacts to such inflammatory processes. Transauricular vagus nerve stimulation (tVNS), a peripheral and minimally invasive neuromodulation approach, modulates multiple areas of the brain by activating reflex circuits in the central nervous system. The purpose of this study was to investigate the effects of tVNS on pain behavior and inflammation caused by a moderate dosage of lipopolysaccharide (LPS). Mature male Sprague Dawley (180-200 gr) rats were divided into four groups (n=32): sham, tVNS, LPS, and LPS+tVNS. Periorbital pain thresholds were examined by Von Frey Filaments and anxiety was measured by elevated plus maze. tVNS or a sham was carried out for 30 minutes under anesthesia. Rats were sacrificed, and serum samples for ELISA assays were taken. The acute inflammation caused by LPS reduced mobility, decreased Von Frey filament pain thresholds, and increased freezing and anxiety behavior. tVNS reduced freezing but did not reverse pain thresholds or elevated plus maze results. Serum haptoglobulin, IL-6 and CGRP levels were significantly elavated in the LPS group. tVNS did not exert statistically significant changes in aforementioned inflammatory biomarkers. Acute systemic inflammation induced by a single dose of moderate LPS is associated with decreased periorbital pain thresholds and elevated inflammatory biomarkers. A single 30-minute tVNS application had no effect on either circulating inflammatory biomarkers or pain behavioral alterations. This study was supported by Gazi University Scientific Research Projects unit (TOA-2022-8017 and TGA-2022-7857).