A novel benzimidazole and other constituents with antiproliferative and antioxidant properties from Thymelaea microphylla Coss. et Dur


Noman L., ÖKE ALTUNTAŞ F., Zellagui A., YAĞLIOĞLU A. Ş. , DEMİRTAŞ İ., Cardoso S. M. , ...More

NATURAL PRODUCT RESEARCH, vol.31, no.17, pp.2032-2041, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 17
  • Publication Date: 2017
  • Doi Number: 10.1080/14786419.2016.1274888
  • Journal Name: NATURAL PRODUCT RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2032-2041
  • Keywords: Thymelaea microphylla, microphybenzimidazole, antiproliferative activity, HeLa, C6, antioxidant activity, WIKSTROEMIA-INDICA, GLYCOSIDES, APOPTOSIS, AGENTS, CELLS
  • Gazi University Affiliated: Yes

Abstract

A new compound (microphybenzimidazole, 7) along with the six known compounds matairesinol (1), prestegane B (2), umbelliferone (3), daphnoretin (4), microphynolide A (5) and microphynolide B (6) were isolated from Thymelaea microphylla. The structures of the pure compounds were elucidated by spectroscopic and mass-spectrometric analyses, including 1D-, 2D-NMR, and HPLC-TOF/MS. Compounds 2 and 4, as well as three fractions (F6, F6-C5, and F6-W42) obtained from a 50% (v:v) CH2Cl2:MeOH extract exhibited a selective activity against rat brain glioma cells (C6). Moreover, compound 1 and other fractions obtained from 50% (v:v) CH2Cl2:MeOH and 70% (v:v) MeOH:H2O extracts exhibited dose- and time-dependent effects on human cervical cancer cell (HeLa), as measured by xCELLigence assay. Compound 2 (IC50=14.0 +/- 0.2g/mL) and fraction F5 (IC50=12.4 +/- 0.1g/mL) showed higher radical scavenging ability than the synthetic agent butylated hydroxytoluene (BHT, IC50=22.7 +/- 0.6g/mL)