A novel benzimidazole and other constituents with antiproliferative and antioxidant properties from Thymelaea microphylla Coss. et Dur

Noman L., ÖKE ALTUNTAŞ F., Zellagui A., YAĞLIOĞLU A. Ş., DEMİRTAŞ İ., Cardoso S. M., ...Daha Fazla

NATURAL PRODUCT RESEARCH, cilt.31, sa.17, ss.2032-2041, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 17
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1080/14786419.2016.1274888
  • Dergi Adı: NATURAL PRODUCT RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2032-2041
  • Anahtar Kelimeler: Thymelaea microphylla, microphybenzimidazole, antiproliferative activity, HeLa, C6, antioxidant activity, WIKSTROEMIA-INDICA, GLYCOSIDES, APOPTOSIS, AGENTS, CELLS
  • Gazi Üniversitesi Adresli: Evet

Özet

A new compound (microphybenzimidazole, 7) along with the six known compounds matairesinol (1), prestegane B (2), umbelliferone (3), daphnoretin (4), microphynolide A (5) and microphynolide B (6) were isolated from Thymelaea microphylla. The structures of the pure compounds were elucidated by spectroscopic and mass-spectrometric analyses, including 1D-, 2D-NMR, and HPLC-TOF/MS. Compounds 2 and 4, as well as three fractions (F6, F6-C5, and F6-W42) obtained from a 50% (v:v) CH2Cl2:MeOH extract exhibited a selective activity against rat brain glioma cells (C6). Moreover, compound 1 and other fractions obtained from 50% (v:v) CH2Cl2:MeOH and 70% (v:v) MeOH:H2O extracts exhibited dose- and time-dependent effects on human cervical cancer cell (HeLa), as measured by xCELLigence assay. Compound 2 (IC50=14.0 +/- 0.2g/mL) and fraction F5 (IC50=12.4 +/- 0.1g/mL) showed higher radical scavenging ability than the synthetic agent butylated hydroxytoluene (BHT, IC50=22.7 +/- 0.6g/mL)