A novel benzimidazole and other constituents with antiproliferative and antioxidant properties from Thymelaea microphylla Coss. et Dur

Noman L., ÖKE ALTUNTAŞ F. , Zellagui A., YAĞLIOĞLU A. Ş. , DEMİRTAŞ İ., Cardoso S. M. , ...Daha Fazla

NATURAL PRODUCT RESEARCH, cilt.31, sa.17, ss.2032-2041, 2017 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Konu: 17
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1080/14786419.2016.1274888
  • Sayfa Sayıları: ss.2032-2041


A new compound (microphybenzimidazole, 7) along with the six known compounds matairesinol (1), prestegane B (2), umbelliferone (3), daphnoretin (4), microphynolide A (5) and microphynolide B (6) were isolated from Thymelaea microphylla. The structures of the pure compounds were elucidated by spectroscopic and mass-spectrometric analyses, including 1D-, 2D-NMR, and HPLC-TOF/MS. Compounds 2 and 4, as well as three fractions (F6, F6-C5, and F6-W42) obtained from a 50% (v:v) CH2Cl2:MeOH extract exhibited a selective activity against rat brain glioma cells (C6). Moreover, compound 1 and other fractions obtained from 50% (v:v) CH2Cl2:MeOH and 70% (v:v) MeOH:H2O extracts exhibited dose- and time-dependent effects on human cervical cancer cell (HeLa), as measured by xCELLigence assay. Compound 2 (IC50=14.0 +/- 0.2g/mL) and fraction F5 (IC50=12.4 +/- 0.1g/mL) showed higher radical scavenging ability than the synthetic agent butylated hydroxytoluene (BHT, IC50=22.7 +/- 0.6g/mL)