In this study, the effects of the potential application of digestate as an agricultural fertiliser obtained from anaerobically digested microalgae treated by three pretreatment methods, namely alkaline hydrogen peroxide (AHP), high temperature and pressure (HTP), and hydrodynamic cavitation (HC) on some properties of soil, and wheat growth and yield were investigated. For this purpose, pretreated and anaerobically digested microalgae digestates alone or together with diammonium phosphate (DAP) as a chemical fertiliser were applied to soil for wheat growth. The highest dosage of AHP pretreated digestate combined with a half dose of DAP applied to soil was rich in nutrients as 0.25%N and 7.19 mg kg(-1) compared to all groups. The properties of the soils were enhanced by applying the highest dosage (0.06 g kg(-1)) of microalgae digestate combined with a half dose of DAP. 0.02 g kg(-1) dosage of HC pretreated digestate combined with a half dose of DAP also greatly improved nitrogen use efficiency indices by up to 104%. The soils' enzyme activities increased in wheat growth experiments by applying either raw or pretreated microalgae digestates. The soils' beta-glycosidase, alkaline phosphatase, and urease enzyme activities increased to 1.38 mg pNP g(-1) soil, 4.91 mg pNP g(-1) soil, and 2.27 mg NH4-N 100 g(-1) soil respectively by the application of highest dosage of HC pretreated digestate. The digestates did not have a toxic effect on wheat growth, it was determined that applied pretreatment processes did not cause significant changes in wheat plant height or wet and dry weight.