Comprehensive genome analysis of a novel actinobacterium with high potential for biotechnological applications, Nonomuraea aridisoli sp. nov., isolated from desert soil

SAYGIN H., AY H., Guven K., ÇETİN D., ŞAHİN N.

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, cilt.114, sa.12, ss.1963-1975, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 114 Sayı: 12
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1007/s10482-021-01654-z
  • Dergi Adı: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.1963-1975
  • Anahtar Kelimeler: Actinobacteria, Bioactive gene clusters, Carbohydrate-active enzymes, CRISPR-associated sequences, Desert, GENUS NONOMURAEA, ACTINOMYCETE, RECLASSIFICATION, CLASSIFICATION, IMPROVEMENTS, SEQUENCES, BACTERIA, DATABASE, STRAIN, TREES
  • Gazi Üniversitesi Adresli: Evet

Özet

During a study to isolate such actinobacteria with unique metabolic potential, a novel actinobacterium, designated KC333(T), was isolated from a soil sample collected from the Karakum Desert, Turkmenistan. The taxonomic position of the strain was investigated using a polyphasic approach. Phylogenetic analysis of the 16S rRNA gene sequence showed that the strain was most closely related to Nonomuraea terrae CH32(T) (99.0% sequence similarity), Nonomuraea maritima FXJ7.203( T) (98.9%), Nonomuraea candida HMC10(T) (98.7%) and Nonomuraea gerenzanensis ATCC 39727( T) (98.6%), and is therefore considered to represent a member of the genus Nonomuraea. However, the average nucleotide identity and digital DNA-DNA hybridization based on whole-genome sequences between strain KC333(T) and close relatives demonstrated that it represents a novel species of the genus Nonomuraea. The major cellular fatty acids of strain KC333(T) were iso-C-16: 0, C-17:0 10-methyl and iso-C-16: 0 2OH. Strain KC333(T) contained meso-diaminopimelic, mannose, madurose and ribose in the cell-wall peptidoglycan. The predominant menaquinones were MK-9(H-4) and MK-9(H-6). The genome size of strain KC333(T) is approximately 9.86 Mb, and the genomic DNA G + C content of the strain is 71.3%. In addition to the polyphasic characterisation, comprehensive genome analysis for gene clusters encoding carbohydrate-active enzymes and bioactive secondary metabolites as well as CRISPR-associated sequences revealed the high biotechnological potential of the strain. Based on evidence collected from the genotypic, phenotypic, and phylogenetic analyses, a novel species, Nonomuraea aridisoli sp. nov. is proposed with KC333(T) (= DSM 107062( T) = JCM 32584( T) = KCTC 49111( T)) as the type strain.