Visualization of sperm by immunohistochemistry: Application of alternative counterstains

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Erdogan D., Elmas Ç., Ilgaz C.

Archives of Andrology, vol.51, no.3, pp.233-238, 2005 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 51 Issue: 3
  • Publication Date: 2005
  • Doi Number: 10.1080/014850190884246
  • Journal Name: Archives of Andrology
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.233-238


The aim of this study was to evaluate the application of hematoxylin, safranin, light green and picric acid as counterstain in sperm immunohistochemistry. This is important to visualize the best staining procedure and to determine the advantage of picric acid as a counterstain in some situations. Picric acid used for counterstaining in the immunohistochemical procedure gives the best image of reaction on sperms when DAB was used as a chromogen.