Anodic Oxidation of Cladribine and In Situ Evaluation of DNA-Cladribine Interaction


Pontinha A. D. R. , Satana H. E. , Diculescu V. C. , Oliveira-Brett A. M.

ELECTROANALYSIS, vol.23, no.11, pp.2651-2657, 2011 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 23 Issue: 11
  • Publication Date: 2011
  • Doi Number: 10.1002/elan.201100320
  • Title of Journal : ELECTROANALYSIS
  • Page Numbers: pp.2651-2657

Abstract

Cladribine (CLD), 2-chlorodeoxyadenosine, was investigated at a glassy carbon electrode using cyclic, differential pulse and square wave voltammetry over a wide pH range. The oxidation of CLD is an irreversible, pH-dependent with the transfer of two electrons and two protons mechanism, leading to the formation of a hydroxylated species which undergoes reversible redox reactions with the transfer of one electron and one proton. The in situ evaluation of DNA-CLD interaction using a DNA-electrochemical biosensor was investigated. The CLD interaction causes dsDNA structural modifications in a time-dependent manner, but no DNA oxidative damage caused by CLD was observed.