Anodic Oxidation of Cladribine and In Situ Evaluation of DNA-Cladribine Interaction


Pontinha A. D. R., Satana H. E., Diculescu V. C., Oliveira-Brett A. M.

ELECTROANALYSIS, vol.23, no.11, pp.2651-2657, 2011 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 23 Issue: 11
  • Publication Date: 2011
  • Doi Number: 10.1002/elan.201100320
  • Journal Name: ELECTROANALYSIS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2651-2657
  • Keywords: Cladribine, DNA, Oxidation, Oxidative damage, 2-CHLORO-2'-DEOXYADENOSINE, LEUKEMIA, 2-CDA
  • Gazi University Affiliated: Yes

Abstract

Cladribine (CLD), 2-chlorodeoxyadenosine, was investigated at a glassy carbon electrode using cyclic, differential pulse and square wave voltammetry over a wide pH range. The oxidation of CLD is an irreversible, pH-dependent with the transfer of two electrons and two protons mechanism, leading to the formation of a hydroxylated species which undergoes reversible redox reactions with the transfer of one electron and one proton. The in situ evaluation of DNA-CLD interaction using a DNA-electrochemical biosensor was investigated. The CLD interaction causes dsDNA structural modifications in a time-dependent manner, but no DNA oxidative damage caused by CLD was observed.