Wound healing and anti-inflammatory effects of aerial parts and roots of S. acuminata, S. cana var. alpina, S. cana var. jacquiniana, S. cana var. radicosa, S. eriophora, S. laciniata ssp. laciniata, S. suberosa ssp. suberosa and S. sublanata were investigated in current study to clarify the traditional usage of Scorzonera species growing in Turkey. It is well known that some species of the Scorzonera genus are used for wound healing in Turkish and European traditional medicine. Therefore, wound healing effect of the plants was assessed by using linear incision and circular excision experimental wound models and subsequently histopathological analysis. Hydroxyproline content of the treated tissues was also assessed. Furthermore, the extracts were screened for anti-hyaluronidase activity. For the evaluation of anti-inflammatory activity, acetic acid-induced increase in capillary permeability test was used. 20% aqueous methanol extracts of the aerial parts of Scorzonera species, S. cana var. jacquiniana and S. eriophora were found to be effective on the wound and anti-inflammatory activity test models. The results of histopathological examination supported the outcome of linear incision and circular excision wound models. Phytochemical analyses of the tested extracts were also performed by using developed and validated HPLC method. Among the tested standard compounds, varying amounts of the chlorogenic acid, rutin, hyperoside and luteolin-7-glycoside were determined in Scorzonera species.