In this study, a series of semi-interpenetrating polymer network (IPN) hydrogels were prepared as a support material for lipase immobilization. Hydrogels were synthesized via free radical polymerization in different compositions of chitosan (Cs), acrylamide (AAm), and citraconic acid (CA). The swelling values of the hydrogels were found to be 240-400%. Depending on the swelling results, Cs-P(AAm-co-CA)-2 hydrogel was chosen for lipase immobilization. Three different types of immobilization technique were carried out. Lipase release behaviors were investigated, and immobilization yields of three immobilization methods were compared, and the maximum immobilization yield value was determined for entrapment method.