Simultaneous determination of valsartan and hydrochlorothiazide in tablets by first-derivative ultraviolet spectrophotometry and LC

Satana E. , Altinay S., Goger N. , Ozkan S., Senturk Z.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, vol.25, pp.1009-1013, 2001 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 25
  • Publication Date: 2001
  • Doi Number: 10.1016/s0731-7085(01)00394-6
  • Page Numbers: pp.1009-1013


First-derivative ultraviolet spectrophotometry and high-performance liquid chromatography (HPLC) were used to determine valsartan and hydrochlorothiazide simultaneously in combined pharmaceutical dosage forms. The derivative procedure was based on the linear relationship between the drug concentration and the first derivative amplitudes at 270.6 and 335 nm for valsartan and hydrochlorothiazide respectively. The calibration graphs were linear in the range of 12.0-36.1 mug ml(-1) for valsartan and 4.0-12.1 mug ml(-1) for hydrochlorothiazide. Furthermore, a highperformance liquid chromatographic procedure with ultraviolet detection at 22 nm was developed for a comparison method. For the HPLC procedure, a reversed phase column with a mobile phase of 0.02 M phosphate buffer (pH 3.2)-acetonitrile (55: 45; v/v). was used to separate for valsartan and hydrochlorothiazide. The plot of peak area ratio of each drug to the internal standard versus the respective concentrations of valsartan and hydrochlorothiazide were found to be linear in the range of 0.06-1.8 and 0.07-0.5 mug m(-1), respectively. The proposed methods were successfully applied to the determination of these drugs in laboratory-prepared mixtures and commercial tablets. (C) 2001 Elsevier Science B.V. All rights reserved.