Production of L-DOPA using Cu-alginate gel immobilized tyrosinase in a batch and packed bed reactor


Ates S., Cortenlioglu E., Bayraktar E., Mehmetoglu U.

ENZYME AND MICROBIAL TECHNOLOGY, cilt.40, sa.4, ss.683-687, 2007 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 40 Konu: 4
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1016/j.enzmictec.2006.05.031
  • Dergi Adı: ENZYME AND MICROBIAL TECHNOLOGY
  • Sayfa Sayıları: ss.683-687

Özet

In this study, production of L-DOPA (L-3, 4-dihydroxy phenylalanine) was investigated by using tyrosinase enzyme in batch and packed bed reactors. Tyrosinase has been immobilized with method of entrapment in copper-alginate gels. L-DOPA concentration obtained from batch reactor for free and immobilized enzyme was 9.5 and 4.5 mg/L, respectively. L-DOPA concentration was obtained as 1.2 mg/L in the packed bed reactor. When air was introduced L-DOPA production increased 6.4 times in packed bed reactor and the productivity was calculated as 110mg/Lh. (c) 2006 Elsevier Inc. All rights reserved.