Production of L-DOPA using Cu-alginate gel immobilized tyrosinase in a batch and packed bed reactor

Ates S., Cortenlioglu E., Bayraktar E., Mehmetoglu U.

ENZYME AND MICROBIAL TECHNOLOGY, vol.40, no.4, pp.683-687, 2007 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 40 Issue: 4
  • Publication Date: 2007
  • Doi Number: 10.1016/j.enzmictec.2006.05.031
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.683-687
  • Keywords: L-DOPA production, tyrosinase, immobilized enzymes, packed bed reactor, copper alginate gel
  • Gazi University Affiliated: No


In this study, production of L-DOPA (L-3, 4-dihydroxy phenylalanine) was investigated by using tyrosinase enzyme in batch and packed bed reactors. Tyrosinase has been immobilized with method of entrapment in copper-alginate gels. L-DOPA concentration obtained from batch reactor for free and immobilized enzyme was 9.5 and 4.5 mg/L, respectively. L-DOPA concentration was obtained as 1.2 mg/L in the packed bed reactor. When air was introduced L-DOPA production increased 6.4 times in packed bed reactor and the productivity was calculated as 110mg/Lh. (c) 2006 Elsevier Inc. All rights reserved.