A new efficient method for eliminating the interference effect of human serum and increasing the sensitivity and recovery rate of enzyme immunoassay

Karakus R., Buyrukcu B., Aybay C.

JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY, cilt.26, sa.2, ss.109-124, 2005 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26 Sayı: 2
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1081/ias-200051994
  • Dergi Adı: JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.109-124
  • Gazi Üniversitesi Adresli: Evet

Özet

A new, very simple method for increasing the sensitivity and recovery rate of enzyme-linked immunosorbent assay (ELISA) for the precise quantification of antigen in human serum is described. The assay design uses CATNF6A4c IgG2a monoclonal antibody and biotinylated anti-human tumor necrosis factor-alpha (hTNF-alpha) polyclonal mouse IgG as the capture and tracer antibodies, respectively.