A new efficient method for eliminating the interference effect of human serum and increasing the sensitivity and recovery rate of enzyme immunoassay

Karakus, RESUL; Buyrukcu, BA; Aybay, C

doi:10.1081/ias-200051994

A new efficient method for eliminating the interference effect of human serum and increasing the sensitivity and recovery rate of enzyme immunoassay

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Karakus R., Buyrukcu B., Aybay C.

JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY, vol.26, no.2, pp.109-124, 2005 (SCI-Expanded)

Publication Type: Article / Article
Volume: 26 Issue: 2
Publication Date: 2005
Doi Number: 10.1081/ias-200051994
Journal Name: JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY
Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Page Numbers: pp.109-124
Gazi University Affiliated: Yes

Abstract

A new, very simple method for increasing the sensitivity and recovery rate of enzyme-linked immunosorbent assay (ELISA) for the precise quantification of antigen in human serum is described. The assay design uses CATNF6A4c IgG2a monoclonal antibody and biotinylated anti-human tumor necrosis factor-alpha (hTNF-alpha) polyclonal mouse IgG as the capture and tracer antibodies, respectively.