Properties of immobilized glucose oxidase and enhancement of enzyme activity


Ates S., Icli N.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, vol.41, no.4, pp.264-268, 2013 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 41 Issue: 4
  • Publication Date: 2013
  • Doi Number: 10.3109/10731199.2012.731415
  • Journal Name: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.264-268
  • Keywords: calcium alginate gel, gluconic acid, immobilized glucose oxidase, silicone oil, soybean oil, ACID PRODUCTION
  • Gazi University Affiliated: Yes

Abstract

In this study; glucose oxidase (GOD) and MnO2 were immobilized in alginate gel beads using the entrapment method and effects of oxygen vectors on immobilized GOD activity were investigated. The specific activity of free enzyme was 1.3 units/mg protein whereas that of immobilized enzyme was 8.4 x 10(-1) units/mg protein. The Km values for free and immobilized enzyme were found to be 2.7 mM, 2.9 mM; Vmax values 3.64 x 10(-1) mu mol/min.mL, 2.61 x 10(-1) mu mol/min.mL, respectively. When 4% (v/v) silicone oil or soybean oil was used, the activity of immobilized GOD increased 1.7 times and 1.3 times, respectively, compared to media without these oils.