Bacopa Monnieri Protects the Directly Affected Organ as Well as Distant Organs Against I/R Injury by Modulating Anti-Inflammatory and Anti-Nitrosative Pathways in A Rat Model for Infra-Renal Aortic Occlusion

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Ozlu H., Cakir Gundogdu A., Elmazoglu Z., Take Kaplanoğlu G., Oktar L., Karasu Ç.

JOURNAL OF INVESTIGATIVE SURGERY, cilt.34, sa.9, ss.935-946, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34 Sayı: 9
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1080/08941939.2020.1716118
  • Dergi Adı: JOURNAL OF INVESTIGATIVE SURGERY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, EBSCO Legal Collection, EBSCO Legal Source, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.935-946
  • Anahtar Kelimeler: Bacopa monnieri, ischemia-reperfusion, infra-renal aortic occlusion, nitrosative stress, inflammation, apoptosis, ISCHEMIA-REPERFUSION INJURY, IN-VITRO, CATALASE, MITOCHONDRIAL, APOPTOSIS, LUTEOLIN, PLANT
  • Gazi Üniversitesi Adresli: Evet

Özet

Objective: To investigate the protective effect and underlying mechanisms of B. monnieri, a medicinal plant, on kidney and skeletal muscle injury induced by infra-renal abdominal aorta clamping for 2-hours (ischemia) and following removal of the clamp (reperfusion, 2-hours). Methods: Rats were divided into four groups (n = 6): (I) animals given only saline (sham-control); (II) animals given B. monnieri extract for 10-days (300 mg/kg/day) (Bacopa-treated sham); (III) animals subjected to ischemia/reperfusion (I/R); (IV) animals given B. monnieri extract and then subjected to I/R. Kidneys and lower extremity muscles were examined for GPx, CAT, iNOS, 3-NT, IL-1 beta and TNF-alpha. Apoptosis and injury were evaluated by TUNEL and H&E staining, respectively. Results: I/R resulted in TUNEL positive cells, periarterial edema and glomerular capillary dilatation, decreased GPx activity, unchanged CAT, iNOS, 3-NT, IL-1 beta and TNF-alpha in kidney. B. monnieri minimized renal remote reperfusion injury, and Group IV showed a lower degree of renal histopathology score when compared to the others. B. monnieri mitigated muscle I/R injury, decreased muscle hypertrophy, myofibril abnormalities and apoptosis. Muscle 3-NT and cytokine levels were increased by I/R, and B. monnieri inhibited iNOS and 3-NT both in sham-control and I/R groups. Muscle GPx unaffected by I/R or B. monnieri, but CAT was inhibited only in B. monnieri-treated I/R group. Muscle iNOS, 3-NT, IL-1 beta, TNF-alpha levels and CAT activity of B. monnieri-treated I/R rats were lower than those in sham-control or Bacopa-treated sham. Conclusions: B. monnieri can protect the directly affected organ as well as distant organs against I/R injury by modulating anti-inflammatory and anti-nitrosative pathways.