Expression patterns of miR-221 and its target Caspase-3 in different cancer cell lines

Ergun S., Arman K., Temiz E., Bozgeyik I., Yumrutas O., Safdar M., ...More

MOLECULAR BIOLOGY REPORTS, vol.41, no.9, pp.5877-5881, 2014 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 41 Issue: 9
  • Publication Date: 2014
  • Doi Number: 10.1007/s11033-014-3461-6
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.5877-5881
  • Keywords: MiR-221, Caspase-3, Apoptosis, Cancer cell line, MICRORNA EXPRESSION, PROSTATE, ACTIVATION, P27(KIP1), CARCINOMA
  • Gazi University Affiliated: No


Caspases are important initiators and most well-known finishers of apoptosis. By changing the death propagation homeostatic equilibrium, their different expression patterns might trigger the progression of hazardous diseases like cancer. miR-221 is an oncogenic miRNA. It is known to have both anti-angiogenic and angiogenic effect. The aim of this work was to compare the expression levels of miR-221 and its target caspase-3 in different cancer cell lines and to find out a relationship between these two. We also tried to establish a prominent relationship between miR-221 and its role in apoptosis by studying their expression levels. Our results indicate that expression of caspase-3 is quite lower as compared to miR-221 expression in all of the selected cancer cell lines. As a result, we conclude that miR-221 may have a crucial role in repressing the expression of caspase-3 which may contribute to a lower apoptotic rate, thus supporting the selection of more aggressive cancer cells. To our knowledge, this is the first study related to the expression levels of caspase-3 and miR-221 in different cell lines at the same time. We expect that our study might pave the way for better understanding the role of miR-221 in apoptotic regulation of caspase-3.