In Vitro Evaluation of Cytotoxicity of Soft Lining Materials on L929 Cells by MTT Assay

Ozdemir K. G., YILMAZ H., Yilmaz S.

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, sa.1, ss.82-86, 2009 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1002/jbm.b.31256
  • Dergi Adı: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.82-86
  • Anahtar Kelimeler: cytotoxicity, soft lining material, L929, MTT assay, RESIN COMPONENTS, DENTAL MATERIALS, LONG-TERM, FIBROBLASTS, BIOCOMPATIBILITY, LINERS, PLASTICIZERS, TOXICITY, EXPOSURE, DURATION
  • Gazi Üniversitesi Adresli: Evet

Özet

The aim of this in vitro study was to evaluate five commonly used soft lining materials, Viscogel (VG), Ufi Gel P (UGP), Softliner (S), Coe-Soft (CS), and Molloplast-B (MB) in terms of cytotoxicity by MTT assay, using L929 mouse fibroblasts. Sixteen disk-shaped specimens from each material were prepared (according to the manufacturer's instructions) in stainless steel mold (10 mm diameter and 1.5 mm thick). The specimens were incubated for 24, 48, 72, and 96 h in Dulbecco's Modified Eagle Medium Ham's F12 (DMEM/F12) and following each incubation interval, cytotoxicity of the extracts to cultured mouse fibroblasts (L929) were measured by MTT assay. Data were statistically analyzed by two-way analysis of variance (ANOVA), and Duncan's test, at a significance level of p < 0.05. Group CS revealed significantly high cytotoxic effect at all incubation periods (p < 0.05). Although no cytotoxic effect for Group S was found at 24, 48, 72 h periods, it has been raised at 96-h incubation period (p > 0.05). Group VG, UGP, S (except at 96 h period), and Group MB demonstrated high cell survival rates at incubation periods. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 90B: 82-86. 2009