Akademik Veri Yönetim Sistemi
Colloids and Surfaces A: Physicochemical and Engineering Aspects, cilt.685, 2024 (SCI-Expanded)
Reactive polymeric thin films designed on solid surfaces have widespread applications in many fields involving the immobilization of biomolecules. Efficient and specific designs for biomolecule immobilization on polymer-modified solid surfaces are central to biosensor technologies such as protein and gene chips. Research areas related to the functionalization of surfaces include polymer brushes whose functionality is increased by the Diels-Alder (DA) reaction, which have been made usable as rewritable platforms with their thermal transformation properties and have found a lot of use, especially in biomolecule determination studies. Glutathione (GSH), one of the biomolecules, is found in in very high intracellular concentrations. It is the most important antioxidant molecule in the environment and its determination is a good indicator for us to obtain information about the status of the antioxidant defense mechanism in various diseases. When studies in were examined in detail, no study was found on the synthesis of polymer brushes with thiol functional groups covalently bonded to the silicon surface using the interfacial-mediated RAFT polymerization technique and their use in GSH adsorption. Therefore, in this study, to fill this gap in the literature, polymer brushes with thiol (SH) functional groups to be used in GSH adsorption were synthesized. Poly(furfurylmethacrylate) [poly(FMA)] brushes, formed by polymer chains covalently bonded to the silicon surface, were synthesized by interface-mediated RAFT polymerization. Then, by maleimide conjugation using hexylamine, polymeric brushes with thiol functional groups covalently bonded to the silicon surface which can be used in GSH adsorption were obtained for determination purposes. It is thought that the prepared polymeric brushes will find a wide application area for the determination of many new molecules, especially the determination of glutathione and will contribute to the literature.