Comparison of the methods evaluating post thawing viability of peripheral blood stem cell graft

Varan H., Bay M., Ozturk A., Dalva K., Ilhan O.

TRANSFUSION AND APHERESIS SCIENCE, cilt.58, sa.2, ss.192-195, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 58 Sayı: 2
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1016/j.transci.2019.03.016
  • Dergi Adı: TRANSFUSION AND APHERESIS SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.192-195
  • Anahtar Kelimeler: Viability, Stem cell, Blood, Postthawing, ACRIDINE-ORANGE, TRYPAN BLUE, CRYOPRESERVATION, CD34(+), SINGLE, IMPACT
  • Gazi Üniversitesi Adresli: Hayır

Özet

Objective: Reliable and pratique methods are essential for rapid and accurate determination of post thawing viability of peripheral blood stem cell (PBSC) graft before hematopoietic stem cell transplantation. In this study, Trypan Blue (TP) Eosin Y (EO), and Acridine-orange-ethidium bromide (AO/EB), which are of the methods commonly used for the assessment of viability in clinic practice, were compared with the flow cytometry-7AAD (7AAD) method, which is a more sensitive method. The aim of this study is to examine which method evaluates postthawing viability in a more compatible manner with 7AAD.