Determination of antazoline and tetrahydrozoline in ophthalmic solutions by capillary electrophoresis and stability-indicating HPLC methods


GÜMÜŞTAŞ M., Alshana U., ERTAŞ N. , GÜNDEN GÖĞER N. , ÖZKAN S. A. , USLU B.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, cilt.124, ss.390-398, 2016 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 124
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1016/j.jpba.2016.02.032
  • Dergi Adı: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
  • Sayfa Sayıları: ss.390-398

Özet

Capillary electrophoretic (CE) and high performance liquid chromatographic (HPLC) methods were developed and optimized for the determination of antazoline (ANT) and tetrahydrozoline (TET) in ophthalmic formulations. Optimum electrophoretic conditions were achieved using a background electrolyte of 20 mM phosphate buffer at pH 7.0, a capillary temperature of 25 degrees C, a separation voltage of 22 kV and a pressure injection of the sample at 50 mbar for 17 s. HPLC analysis was performed with Kinetex (150 x 4.6 mm ID x 5 mu m) (Phenomenex, USA) analytical column with 1 mL min(-1) flow rate of mobile phase which consisted of 0.05% TFA in bidistilled water (pH adjusted to 3.0 with 5 M NaOH) and acetonitrilelbuffer in the ratio of 63:37 (v/v) at room temperature. Injection volume of the samples was 10 mu L and the wavelength of the detector was set at 215 nm for monitoring both analytes.