Effects of Bacillus thuringiensis kurstaki on midgut cells of Thaumetopoea pityocampa (Lepidoptera: Thaumetopoeidae), the pine processionary caterpillar


UZUNHİSARCIKLI M., KALENDER Y., Suludere Z.

Gazi University Journal of Science, cilt.27, sa.2, ss.861-870, 2014 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Sayı: 2
  • Basım Tarihi: 2014
  • Dergi Adı: Gazi University Journal of Science
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.861-870
  • Anahtar Kelimeler: Bacillus thuringiensis kurstaki, Midgut, Thaumetopoea pityocampa, Transmission electron microscope, Ultrastructure
  • Gazi Üniversitesi Adresli: Evet

Özet

In this study effects of Bacillus thuringiensis kurstaki (Btk) on midgut cells of Thaumetopoea pityocampa larvae was investigated by electron microscopy. 3 mg/1 Btk was given with food. 1 h, 3 h and 6 h after Btk administration no pathological changes in the midgut epitelial cells were observed. 12 h after Btk administration swelling of mitochondria in midgut epitelial cells were observed. Formation of vacuoles in cytoplasm was seen after 24 h. After 48 h decreasing of microvilli of midgut epithelial cell's and partial dissolving of cytoplasm, dissolving of nucleoplasm and chromatin clumps were observed. 60 h after Btk administration swelling and dissolving of mitochondria in midgut epithelial cells were detected. 96 h after Btk administration a membrane-body and dissolving in the cytoplasm clumps in midgut cells of T. pityocampa larvae were occurred. Present study shows that Btk is effective to T. pityocampa larvae.