7th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2024), Ankara, Türkiye, 2 - 04 Ekim 2024, ss.192
Amino acids play a crucial role in numerous biological processes, including protein synthesis, enzyme and hormone production, immune system modulation, neurotransmitter synthesis, energy production, and tissue repair. They also exhibit protective effects against infections and tumour cells due to their antimicrobial, antioxidant, anticancer and immunomodulatory properties. Therefore, in this study, we aimed to determine the antimicrobial, antibiofilm, and anticancer effect of L-Tyrosine amino acid that is a precursor to several important neurotransmitters, including dopamine. The antimicrobial activities and biofilm inhibitions against Candida tropicalis T26, Proteus mirabilis U15, and Staphylococcus epidermidis W17 clinical strains were evaluated using the Broth Dilution and Crystal Violet Binding assays respectively. Meanwhile, the cytotoxicity of L-Tyrosine on HeLa and MDA-MB-231 cell viabilities was determined through the MTT assay. According to our results, L-Tyrosine did not cause any antimicrobial effect against any of the tested strains with a Minimum Inhibition Concentration (MIC) and Minimum Microbicidal Concentration (MMC) values > 200 mg/mL. However, 0.5MIC of L-Tyrosine caused inhibitions against biofilm formation of all the tested clinical isolates, with the highest inhibition observed against S. epidermidis W17 (89.75 %). Cell viability results showed that in both cancer cell types, viability at 24 hours decreased significantly starting from the lowest concentration of 0.01 mg/mL. As the concentration increased, the number of dead cells significantly increased compared to the control (p<0.05). Additionally, a statistically significant decrease in cell viability was observed with increasing concentrations in the 48-hour data compared to the control (p<0.05). Moreover, compared to the MDA_MB_231 cell line, cell viability was more effectively suppressed in HeLa cells. The findings of this study will contribute to the development of anti-biofilm and anti-cancer strategies focused on L-Tyrosine synthesizable molecules. Nonetheless, it would be beneficial to conduct comprehensive experimental and clinical trials as well. Keywords: Antibiofilm effect, Anticancer activity, Candida tropicalis, Cytotoxicity, Proteus mirabilis, Staphylococcus epidermidis