Hesperis muglensis, and H. zaferi collected from Mu??la and ??orum, Turkey, have been defined as new species for the scientific world. Hesperis muglensis grows with H. pisidica calcareous locations in the south of Turkey (Taurus Mountains), while H. zaferi grows glades in forest in Tosya, Kastamonu Province, and in S??t????ler, Isparta Province, and displays a similarity to H. tosyaensis and H. ozcelikii. The photographs, general ecological preferences and of the newly defined species have been given.