Selective Binding of Inhibitor-Assisted Surface-Imprinted Core/Shell Microbeads in Protein Mixtures

Dinc M., BASAN H., Hummel T., Mueller M., Sobek H., Rapp I., ...Daha Fazla

CHEMISTRYSELECT, cilt.3, sa.16, ss.4277-4282, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 3 Sayı: 16
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1002/slct.201800129
  • Dergi Adı: CHEMISTRYSELECT
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.4277-4282
  • Anahtar Kelimeler: Surface chemistry, Molecular recognition, Core-shell microbeads, Inhibitor-assisted, Protein imprinting, POLYMERS, RECOGNITION, ANTIBODIES, RECEPTORS, PEPSIN
  • Gazi Üniversitesi Adresli: Evet

Özet

An innovative approach for molecularly imprinting proteins, i.e., inhibitor-assisted imprinting was used for addressing the major challenge of protein purification from biotechnologically relevant media. Pepstatin-assisted pepsin surface-imprinted microbeads were synthesized as a selective sorbent for solid phase extraction (SPE). The amino-functionalized porous core was prepared by co-condensation and post-grafting strategies. The immobilized inhibitor pre-organizes the template pepsin with a defined orientation prior to imprinting ensuring enhanced efficiency of the imprinting process. 3-aminophenylboronic acid (APBA) was used as a functional monomer establishing organic nanoscale films comprising poly(3-aminophenylboronic acid) (pAPBA) within the pores of the beads in the presence (MIP) and absence (NIP) of pepsin. Next to a detailed characterization of these advanced functional hybrid materials it was demonstrated that such porous surface-imprinted core/shell beads feature exquisite selectivity for pepsin during individual protein rebinding studies, and more importantly, during competitive rebinding studies in protein mixtures.