Isolation of anti-ulcerogenic sesquiterpene lactones from Centaurea solstitialis L. ssp solstitialis through bioassay-guided fractionation procedures in rats

Yesilada E., Gurbuz İ., Bedir E., Tatli I., Khan W.

JOURNAL OF ETHNOPHARMACOLOGY, vol.95, no.2-3, pp.213-219, 2004 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 95 Issue: 2-3
  • Publication Date: 2004
  • Doi Number: 10.1016/j.jep.2004.07.021
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.213-219
  • Keywords: 13-acetyl solstitialin A, anti-ulcer activity, Asteraceae, Centaurea solstitialis ssp solstitialis, chlorojanerin, ethanol-induced ulcerogenesis, solstitialin A, peptic ulcer, sesquiterpene lactone, yellow starthistle, TRADITIONAL MEDICINE, FOLK MEDICINE, TURKEY
  • Gazi University Affiliated: Yes


The fresh spiny flowers of Centaurea solstitialis ssp. solstitialis (CSS) are used for the treatment of peptic ulcers in Turkey. Ethanol (80%) extract of CSS exhibited significant anti-ulcerogenic effect on the ethanol-induced ulcerogenesis model in rats. The ethanol extract was further fractionated by successive solvent extractions with n-hexane, chloroform, ethyl acetate and n-butanol. All fractions showed significant anti-ulcerogenic activity, however, the effect of the chloroform fraction was found to be more prominent with 99.5% ulcer inhibition. Bioassay-guided fractionation yielded sesquiterpene lactones as the active components. The main components responsible for the activity of the chloroform fraction were determined as chlorojanerin and 13-acetyl solstitialin A which were elucidated by HR-ESI and H-1, C-13 and 2D NMR spectroscopic techniques. (C) 2004 Elsevier Ireland Ltd. All rights reserved.