Genotoxic Effects of Fe2O3 Nano- and Microparticles in Lymphocytes by Micronucleus Test

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Özer T., Ünal F., Yüzbaşıoğlu D.

6th INTERNATIONAL NEW YORK CONGRESS ON HEALTH AND SPORTS SCIENCE, New York, Amerika Birleşik Devletleri, 12 - 14 Kasım 2022, ss.146

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: New York
  • Basıldığı Ülke: Amerika Birleşik Devletleri
  • Sayfa Sayıları: ss.146
  • Gazi Üniversitesi Adresli: Evet

Özet

Since the advantageous properties of magnetic nanoparticles were noticed, investigations have accelerated the use of nanomaterials in medicine. In particular, iron (III) oxide (Fe2O3) nanoparticles (NPs) are preferred intensely due to ease of use, ease of obtaining, physiochemical properties, and compatibility with organic and inorganic substances. Iron (Fe) is one of the most important transition metals and can be involved in biological processes in living organisms. Thus, Fe2O3 has become one of the most widely used metal oxide NPs in biological systems. In this research, the genotoxicity of Fe2O3 NPs and microparticles (MPs) was evaluated by micronucleus test (MN). The MN test is used to determine the frequency of MN, nucleoplasmic bridges (NPBs), nuclear buds (NBUDs), apoptotic and necrotic cells, and cells with single, double, and triple nuclei resulting from damage caused by clastogenic or aneugenic agents. Human peripheral lymphocytes were exposed to concentrations of 125, 250, 500, and 750 μg/mL Fe2O3 NPs and MPs for 48 hours. Control groups were also evaluated for negative, sterile distilled water, and for positive, mitomycin-C (MMC). Test results revealed that both of the particles increased the frequency of MN in human lymphocytes. However, this increase was significant in only one dose (250 μg/mL) for both. In addition, both of the particles caused an increase in the frequency of NBUDs and NPBs (except 125 μg/mL; NPs). Increasing at 125, 250, and 500 μg/mL and 250 μg/mL Fe2O3 MPs was significant compared to the control, respectively. These results suggest that Fe2O3 MPs were more effective in the formation of NBUDs and NPBs compared to Fe2O3 NPs in lymphocytes in vitro. These data might have resulted from the aggregation of NPs that can not pass through the cell membrane or the nucleus and therefore can not interact with biological systems. However, more investigations should be carried out for the evaluation of the genotoxic potentials of NPs and MPs.