Akademik Veri Yönetim Sistemi
Internation Joint Science and materials polimer (ICMPS), 14 Ocak 2021
In this study, a new amperometric biosensor for creatinine determination was developed. For this purpose, a
polypyrrole-polyvinylsulfonate (PPy-PVS) film was prepared by electropolymerization of pyrrole in a
polyvinylsulfonate medium on a platinum plate. Creatinase and sarcosine oxidase enzymes were immobilized
on polypyrrole-polyvinylsulfonate film by cross-linking with glutaraldehyde. Contact angle images, SEM, and
AFM of enzyme and non-enzyme surfaces were taken to determine the morphological and chemical properties
of the Pt/PPy-PVS electrode. The determination of creatinine was made based on the oxidation of hydrogen
peroxide at 0.4 V formed as a result of the enzymatic reaction on the surface of the prepared biosensor. The line
ar working range of the biosensor obtained was found between 5.0×10-6 M and 1.0×10-4 M. Using this linear
graph, the Km (observed) and Imax (observed) values for the double enzyme electrode system werecalculated
as 5.0×10-3 mM and 0.34 µA/min, respectively. Optimum working pH and temperature weredetermined as 8.0
and 60 °C, respectively. The reusability and shelf life of the biosensor were determined. The effects of
interferences in biological environments on biosensor response were investigated. For this purpose, uric acid,
ascorbic acid, paracetamol, glycine, urea, and formaldehyde was used. The results have shown that the
prepared biosensor has the potential to be used for creatinine determination in biological fluids.
Keywords: Biological Fluid, Biosensor, Creatinine, Polypyrrole, Polyvinylsulfonate, Sarcosine Oxidase