Design of a new biosensor platform for creatinine determination


Akın Anakök D., Çete S.

Internation Joint Science and materials polimer, Burdur, Turkey, 29 September - 01 October 2021, pp.97

  • Publication Type: Conference Paper / Summary Text
  • City: Burdur
  • Country: Turkey
  • Page Numbers: pp.97
  • Gazi University Affiliated: Yes

Abstract

In this study, a new amperometric biosensor for creatinine determination was developed. For this purpose, a polypyrrole-polyvinylsulfonate (PPy-PVS) film was prepared by electropolymerization of pyrrole in a polyvinylsulfonate medium on a platinum plate. Creatinase and sarcosine oxidase enzymes were immobilized on polypyrrole-polyvinylsulfonate film by cross-linking with glutaraldehyde. Contact angle images, SEM, and AFM of enzyme and non-enzyme surfaces were taken to determine the morphological and chemical properties of the Pt/PPy-PVS electrode. The determination of creatinine was made based on the oxidation of hydrogen peroxide at 0.4 V formed as a result of the enzymatic reaction on the surface of the prepared biosensor. The line ar working range of the biosensor obtained was found between 5.0×10-6 M and 1.0×10-4 M. Using this linear graph, the Km (observed) and Imax (observed) values for the double enzyme electrode system werecalculated as 5.0×10-3 mM and 0.34 µA/min, respectively. Optimum working pH and temperature weredetermined as 8.0 and 60 °C, respectively. The reusability and shelf life of the biosensor were determined. The effects of interferences in biological environments on biosensor response were investigated. For this purpose, uric acid, ascorbic acid, paracetamol, glycine, urea, and formaldehyde was used. The results have shown that the prepared biosensor has the potential to be used for creatinine determination in biological fluids. Keywords: Biological Fluid, Biosensor, Creatinine, Polypyrrole, Polyvinylsulfonate, Sarcosine Oxidase