Akademik Veri Yönetim Sistemi
7th INTERNATIONAL ISTANBUL CURRENT SCIENTIFIC RESEARCH CONGRESS, İstanbul, Türkiye, 13 - 15 Şubat 2026, ss.286-287, (Özet Bildiri)
Alzheimer’s disease (AD) is one of the leading causes of dementia and is a multifactorial, age-related neurodegenerative disorder characterized by memory loss and progressive cognitive decline. Currently available pharmacological treatments for AD only alleviate symptoms, and no curative therapeutic agent has yet been developed. In recent years, advances in biomedical applications have made magnetic nanoparticles (MNPs) promising candidates for the treatment and imaging of central nervous system disorders, particularly in drug delivery and diagnostic applications. Owing to their small size, ability to be guided to target sites under an external magnetic field, and precise control over the concentration and distribution of therapeutic agents, MNPs have begun to replace conventional drug delivery systems. However, various surface coating strategies have been developed to reduce the toxicity and enhance the biocompatibility of MNPs. These approaches enable the design of nanoparticle systems with higher drug-loading capacity, improved stability, and reduced cellular toxicity. Indeed, a previous study reported that surface coating of MNPs with L-exopolysaccharide (LEPS) resulted in the development of nanoparticles exhibiting low toxicity and enhanced biocompatibility. On the other hand, several studies in the literature have demonstrated that certain types of MNPs may induce epigenomic toxicity, including alterations in DNA methylation, histone modifications, and chromatin structure. Therefore, evaluating the epigenetic effects of these L-EPS-coated MNPs, which have been shown to exhibit enhanced bioavailability and significantly reduced cellular toxicity, is of critical importance for determining their safe-use potential. In this study, the effects of L-EPS surface-coated MNPs on histone H3 and H4 acetylation were investigated. Healthy cells and amyloid-β-induced AD model cells were treated with MNPs, EPS, and EPS-coated MNPs, and total H3 and H4 acetylation levels were calculated and compared. The results demonstrated that MNP treatment strongly suppressed histone acetylation, whereas L-EPS coating attenuated the inhibitory effect of MNPs on histone acetylation. Moreover, under amyloid-β stress, L-EPS-coated MNPs markedly increased chromatin accessibility and histone acetylation, shifting the cells toward a distinct epigenetic state. Overall, the findings suggest that L-EPS-coated MNPs may represent a biocompatible biomaterial with epigenetic safety and neuroprotective potential.