Objective: The present study aims to examine investi- gation the -actinin-3 (ACTN3) gene polymorphism (rs1815739) and functional movement screen (FMS) values in young runners competing at the national level. Material and Methods: Forty-five young runners (29 male 64.4%, 16 female 35.6%) competing at the national level in different clubs affiliated to the Turkish Athletic Federation and training regularly at least 5 days a week voluntarily participated in the study. The DNA isolation of oral swab samples was carried out using the spin column (MN Macherey-Nagel, Germany) method. The genotyping pro- cess was performed using the real-time polymerase chain reaction method. Results: The FMS test battery was used to determine the func- tional capacity. According to the findings obtained, it was determined that in the FMS evaluation of the female runners, the runners with the CC genotype had a significantly higher mean score compared to the runners with the CT genotype (p<0.033) while in the male runners, the runners with the CT genotype had a significantly higher mean score compared to the runners with the TT genotype (p<0.031). However, in the FMS evaluation of the overall runners, no significant difference was found (p>0.205). Conclusion: It is thought that the FMS test battery and the ACTN3 gene polymorphism, which is among the important variables responsible for non-impact injuries, can be effective in the level of muscle damage, the type of muscle injury, the prediction of in- juries and reducing the risk factors that may cause injuries.