Evaluation of auricular lymph node cell lymphocyte proliferation and cytokine production as non-radioactive endpoints during murine contact allergy

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ÜLKER Ö., Atak A., ATEŞ İ., Karakaya A.

JOURNAL OF IMMUNOTOXICOLOGY, cilt.8, sa.2, ss.131-139, 2011 (SCI-Expanded)

• Yayın Türü: Makale / Tam Makale
• Cilt numarası: 8 Sayı: 2
• Basım Tarihi: 2011
• Doi Numarası: 10.3109/1547691x.2010.547996
• Dergi Adı: JOURNAL OF IMMUNOTOXICOLOGY
• Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
• Sayfa Sayıları: ss.131-139
• Anahtar Kelimeler: Local lymph node assay, non-radioactive endpoints, DNCB, cytokine production, MOLECULAR-WEIGHT COMPOUNDS, INTERFERON-GAMMA, RESPIRATORY SENSITIZERS, CHEMICAL ALLERGENS, INTEGRATED MODEL, SKIN REACTIONS, ASSAY, MICE, HYPERSENSITIVITY, DIFFERENTIATION
• Gazi Üniversitesi Adresli: Evet

Özet

The murine local lymph node assay (LLNA) has been developed as a test method to assess allergic contact dermatitis. In spite of the validity of the LLNA, attention was drawn to the two disadvantages: use of radioactivity for in vivo measurement of lymph node cell proliferation ([ 1, the applied concentrations of DNCB caused only allergic effect but not any irritant effect. This study reports that the use of these non-radioactive endpoints can assess allergic contact dermatitis caused by chemicals.