Amperometric Detection of Glucose by Polyaniline-Activated Carbon Composite Carbon Paste Electrode

Colak O., ARSLAN H., ZENGİN H., ZENGİN G.

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE, vol.7, no.8, pp.6988-6997, 2012 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 7 Issue: 8
  • Publication Date: 2012
  • Journal Name: INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.6988-6997
  • Gazi University Affiliated: Yes

Abstract

In this study, a novel carbon paste electrode using the salt form of polyaniline (pani)-activated carbon composite sensitive to glucose, was prepared. Glucose oxidase enzyme was immobilized to modified carbon paste electrode by cross-linking with glutaraldehyde. Determination of glucose was carried out by the oxidation of enzymatically produced H2O2 at 0.4 V vs. Ag/AgCl. The effects of pH and temperature were investigated and optimum parameters were found to be 5.0 and 65 degrees C, respectively. The linear working range of the electrode was 5.0x10(-7) - 1.0x10(-5) M, R-2 = 0.980. The storage stability and operation stability of the enzyme electrode were also studied.