Electrochemical biosensor based on glucose oxidase encapsulated within enzymatically synthesized poly(1,10-phenanthroline-5,6-dione)

ÇİFTÇİ H., Oztekin Y., TAMER U., Ramanaviciene A., Ramanavicius A.

COLLOIDS AND SURFACES B-BIOINTERFACES, vol.123, pp.685-691, 2014 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 123
  • Publication Date: 2014
  • Doi Number: 10.1016/j.colsurfb.2014.10.032
  • Journal Name: COLLOIDS AND SURFACES B-BIOINTERFACES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.685-691
  • Keywords: 1,10-Phenanthroline-5,6-dione, Glucose oxidase, Glucose biosensor, Electrocatalysis, Enzymatic polymerization, GOLD NANOPARTICLES, CONDUCTING POLYMER, CARBON NANOTUBES, REDOX ENZYME, IMMOBILIZATION, POLYANILINE, INDICATOR, LAYER, FILMS
  • Gazi University Affiliated: Yes

Abstract

This study is focused on the investigation of electrocatalytic effect of glucose oxidase (GOx) immobilized on the graphite rod (GR) electrode. The enzyme modified electrode was prepared by encapsulation of immobilized GOx within enzymatically formed poly(1,10-phenanthroline-5,6-dione) (pPD) film. The electrochemical responses of such enzymatic electrode (pPD/GOx/GR) vs. different glucose concentrations were examined chronoamperometrically in acetate-phosphate buffer solution (A-PBS), pH 6.0, under aerobic or anaerobic conditions. Amperometric signals of the pPD/GOx/GR electrode exhibited well-defined hyperbolic dependence upon glucose concentration. Amperometric signals at 100 mM of glucose were 41.17 and 32.27 mu A under aerobic and anaerobic conditions, respectively. Amperometric signals of the pPD/GOx/GR electrode decreased by 6% within seven days. The pPD/GOx/GR electrode showed excellent selectivity in the presence of dopamine and uric acid. Furthermore it had a good reproducibility and repeatability with standard deviation of 9.4% and 8.0%, respectively. (C) 2014 Elsevier B.V. All rights reserved.