Akademik Veri Yönetim Sistemi
International Congress on Multidisciplinary Studies-VI Ankara, Ankara, Türkiye, 13 - 14 Ekim 2023, ss.12-23
Methylene blue is
used in many industries such as textile, paper, chemistry and medicine. It is
known that the dye-containing wastewater pollutes the soil and water resources
which it mixes and harms to ecology Synthetic dyes can also cause mutagenic,
toxic, allergic and carcinogenic reactions in humans. Although solutions have
been sought for the removal of these dyes for many years, the search for an
effective, reliable and clean method has led scientists to biological
nanoparticles in recent years. It has been stated in many studies that metal
nanoparticles show high effectiveness in removing pollutants such as heavy
metals, dyes, pesticides, etc. due to their high surface/volume ratios. Nanoparticles can be obtained by chemical and physical
methods. However, in recent years, non-toxic, highly biocompatible alternative
methods have attracted attention. Nanoparticle synthesis
through microorganisms or plants, called green synthesis, has become the focus
of new research because it is environmentally friendly and reliable. Among
metal nanoparticles, silver nanoparticles (AgNp) have a wide range of uses in
medical diagnosis and treatment, controlled drug release, cancer treatments,
preservatives in food packaging and cosmetics, and heavy metal and dye removal.
Studies have shown that different microorganisms such as Actinobacter sp., Bacillus sp, Staphylococcus
aureus, Thiobacillus thioparus, Pseudomonas
aureus, Streptomyces sp.
can synthesize silver nanoparticles. In this study, it was aimed to investigate
the methylene blue removal efficiency of silver nanoparticles obtained by
microorganism-derived green synthesis. Silver nanoparticles were synthesized using Paenibacillus sp. (YSM) bacteria has antimicrobial and cytotoxic
activity. Paenibacillus sp. bacteria and
the bacteria-free supernatant were incubated in 1 mM AgNO3 solution
for 24 hours with shaking (150 rpm) at 37oC. At the end of the
incubation, a brownish color formation specific to silver nanoparticles was
observed in both the pellet and the supernatant. Absorbance scanning of
nanoparticle-containing solutions was performed in the UV-spectrophotometer in
the 325-800 nm wave range. The peaks obtained at 420-450 nm wavelength
confirmed silver nanoparticle synthesis. The obtained AgNps were added to
methylene blue (50 ml, 20 mg) solution and incubated for 72 hours at 37oC
(150 rpm). As a result of the study, it was observed that the nanoparticles
obtained from the pellet had the ability to remove 8% methylene blue, and the
silver nanoparticles obtained from the supernatant had the ability to remove
26%.
Keywords:
Silver nanoparticle,
methylene blue, Paenibacillus sp.,
green synthesis.