Yiyit Doğan S., Kaya S., Yiğit E.

International Congress on Multidisciplinary Studies-VI Ankara, Ankara, Turkey, 13 - 14 October 2023, pp.12-23

  • Publication Type: Conference Paper / Full Text
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.12-23
  • Gazi University Affiliated: Yes


Methylene blue is used in many industries such as textile, paper, chemistry and medicine. It is known that the dye-containing wastewater pollutes the soil and water resources which it mixes and harms to ecology Synthetic dyes can also cause mutagenic, toxic, allergic and carcinogenic reactions in humans. Although solutions have been sought for the removal of these dyes for many years, the search for an effective, reliable and clean method has led scientists to biological nanoparticles in recent years. It has been stated in many studies that metal nanoparticles show high effectiveness in removing pollutants such as heavy metals, dyes, pesticides, etc. due to their high surface/volume ratios. Nanoparticles can be obtained by chemical and physical methods. However, in recent years, non-toxic, highly biocompatible alternative methods have attracted attention. Nanoparticle synthesis through microorganisms or plants, called green synthesis, has become the focus of new research because it is environmentally friendly and reliable. Among metal nanoparticles, silver nanoparticles (AgNp) have a wide range of uses in medical diagnosis and treatment, controlled drug release, cancer treatments, preservatives in food packaging and cosmetics, and heavy metal and dye removal. Studies have shown that different microorganisms such as Actinobacter sp., Bacillus sp, Staphylococcus aureus,  Thiobacillus thioparus, Pseudomonas aureus, Streptomyces sp. can synthesize silver nanoparticles. In this study, it was aimed to investigate the methylene blue removal efficiency of silver nanoparticles obtained by microorganism-derived green synthesis. Silver nanoparticles were synthesized using Paenibacillus sp. (YSM) bacteria has antimicrobial and cytotoxic activity. Paenibacillus sp. bacteria and the bacteria-free supernatant were incubated in 1 mM AgNO3 solution for 24 hours with shaking (150 rpm) at 37oC. At the end of the incubation, a brownish color formation specific to silver nanoparticles was observed in both the pellet and the supernatant. Absorbance scanning of nanoparticle-containing solutions was performed in the UV-spectrophotometer in the 325-800 nm wave range. The peaks obtained at 420-450 nm wavelength confirmed silver nanoparticle synthesis. The obtained AgNps were added to methylene blue (50 ml, 20 mg) solution and incubated for 72 hours at 37oC (150 rpm). As a result of the study, it was observed that the nanoparticles obtained from the pellet had the ability to remove 8% methylene blue, and the silver nanoparticles obtained from the supernatant had the ability to remove 26%.

Keywords: Silver nanoparticle, methylene blue, Paenibacillus sp., green synthesis.