In this study, the amounts of silibinin and rutin compounds found in juniperus excelsa plant fruits were investigated by using sokslet and supercritical CO2 (Sc-CO2) extraction methods. Due to the fact that the juniperus excelsa plant has been used in traditional medicine for centuries for therapeutic purposes and the increase in preventive treatments recently, this has led to intensive research on this plant. In traditional medicine, its diuretic, anti-inflammatory, pain reliever, liver protector, antimicrobial and antibacterial effects are known. Experimental design method was used in the soxhlet extraction of juniperus excelsa fruit, the experiments were designed using the Design Expert 11 (Demo) program and the results were analyzed. Ethanol / water ratio and extraction time were selected as independent parameters and their effects on bioactive material yields was investigated. For the Soxhlet method, mathematical models were obtained that give the maximum amount of silibinin and rutin compounds in the extract. It was observed that the optimum study conditions suggested by the models obtained in the study were compatible with the experimental results. As a result of experimental studies, the highest amounts of silibinin and rutin substances in juniperus excelsa fruits were found as 19.19 mu g/g and 319.6 mu g/g dw, respectively. The silibinin and rutin amounts obtained by Sc-CO2 extraction were found as 17.75 mu g/g and 52.45 mu g/g dw, respectively.