Marsdenia erecta R. Br. is known to have positive effects and is therefore used to treat gastrointestinal disorders, rheumatism, and skin disorders in Turkey. The aim of this study was to investigate the wound healing activity and chemical constituents of wood, inflorescences, leaves, and bark of M erecta. In this study, hydrodistillation and SPME (Solid Phase Microextraction) methods were compared for analysis of essential oils (EO), and chemical corn pounds of the essential oils were determined. The hydrodistillation method revealed components including alpha-pinene, nonanal, safranal in inflorescences; alpha-pinene, theaspirane, 3-hexenyl benzoate in leaves; theaspirane, ethyl palmitate, ethyl linoleate in wood; and eicosane in bark. As a result of SPME analysis, the main compounds detected were alpha-pinene and terpinolene in inflorescences; alpha-pinene, sabinene, 1-oc-ten-3-ol, beta-myrcene, limonene, beta-phellandrene, and cis-sabinene hydrate 4-terpineol in leaves; cyclosativene, alpha-copaene, and beta-caryophyllene in wood; and 3-octanone, beta-copaene, dimethyldecalol, 4-ethylveratrol, and 4-vinylveratrol in bark. In vitro studies showed that the essential oil obtained from wood using the hydrodistillation method inhibits the hyaluronidase and collagenase enzymes and thus promotes the wound healing process.