In Vitro Antioxidant and Cytotoxic Activities of 18 Plants from the Erkowit Region, Eastern Sudan

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Adam M., Elhassan G. O. M., Yagi S., ŞENOL DENİZ F. S., ERDOĞAN ORHAN İ., Ahmed A. A., ...More

NATURAL PRODUCTS AND BIOPROSPECTING, vol.8, no.2, pp.97-105, 2018 (ESCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 8 Issue: 2
  • Publication Date: 2018
  • Doi Number: 10.1007/s13659-018-0155-0
  • Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus
  • Page Numbers: pp.97-105
  • Keywords: Antioxidant activity, Cytotoxicity, Pharmacognosy, Plant extract, OXIDATIVE STRESS, MEDICINAL-PLANTS, RESISTANCE, EXTRACTS, CELLS
  • Gazi University Affiliated: Yes


We investigated the antioxidant potential and cytotoxicity towards human CCRF-CEM leukemia cells of 57 extracts obtained from 18 plants collected in the Erkowit region, eastern Sudan. The antioxidant activity was determined by measuring the radical scavenging effects against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and N, N-dimethyl-p-phenylendiamine (DMPD), metal-chelation capacity, ferric-reducing (FRAP) and phosphomolibdenum-reducing antioxidant power (PRAP) methods using ELISA microtiter assays. Total phenol and flavonoid amounts of the extracts were determined spectrophotometrically. Cytotoxicity towards CCRF-CEM cells was evaluated by the resazurin reduction assay. Geranium favosum followed by Kalanchoe glaucescens, Malva parviflora, Aizoon canariense, and Coleus barbatus, respectively, possessed the highest antioxidant activity among the studied plants. Chrozophora oblongifolia and K. glaucescens exerted considerable cytotoxicity against CCRF-CEM leukemia cells. These plants may serve as source for the further development of natural antioxidant and antitumor agents.