In Vitro Cytotoxicity of Maxillofacial Silicone Elastomers: Effect of Accelerated Aging

TURHAN BAL B., YILMAZ H., AYDIN C., Karakoca S., Yilmaz S.

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, sa.1, ss.122-126, 2009 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1002/jbm.b.31194
  • Dergi Adı: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.122-126
  • Anahtar Kelimeler: cell culture, cytotoxicity, maxillofacial, silicone(s)/PDMS, HUMAN GINGIVAL FIBROBLASTS, CYTO-TOXICITY, ENVIRONMENTAL-FACTORS, PROSTHETIC ELASTOMER, PHYSICAL-PROPERTIES, RESIN COMPONENTS, DENTAL MATERIALS, BIOCOMPATIBILITY, TISSUE, MODELS
  • Gazi Üniversitesi Adresli: Evet

Özet

The purpose of this in vitro study was to evaluate the cytotoxicity of three maxillofacial silicone elastomers at 24, 48, and 72 h on L-929 cells and to determine the effect of accelerated aging on the cytotoxicity of these silicone elastomers. Disc-shaped test samples of maxillofacial silicone elastomers (Cosmesil, Episil, Multisil) were fabricated according to manufacturers' instructions under aseptic conditions. Samples were then divided into three groups: (1) not aged; (2) aged for 150 h with an accelerated weathering tester; and (3) aged for 300 h. Then the samples were placed in Dulbecco's Modified Eagle Medium/Ham's F12 (DMEM/F12) for 24, 48, and 72 h. After the incubation periods, cytotoxicity of the extracts to cultured fibroblasts (L-929) was measured by MTT assay. The degree of cytotoxicity of each sample was determined according to the reference value represented by the cells with a control (culture without sample). Statistical significance was determined by repeated measurement ANOVA (p < 0.01) followed by Duncan's test (p < 0.05). All test materials in each group demonstrated high survival rates in MTT assay (Episil; 93.84%, Multisil; 88.30%, Cosmesil; 87.50%, respectively); however, in all groups, Episil material demonstrated significantly higher cell survival rate after each of the experimental incubation periods (p < 0.05). Accelerated aging for 150 and 300 h had no significant effect on the biocompatibility of maxillofacial silicone elastomers tested (p > 0.05). (C) 2008 Wiley Periodicals. Inc. J Biomed Mater Res Part B: Appl Biomater 89B: 122-126, 2009