Akademik Veri Yönetim Sistemi
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, cilt.25, sa.3, ss.169-174, 2011 (SCI-Expanded)
The aim of this research was to evaluate the acute toxicity of fenitrothion to the crayfish (Astacus leptodactylus Eschscholtz, 1823), which is chosen as an alternative aquatic organism to fish by using the static test system and evaluate the basic lipid peroxidation parameters for the first 24 h. Crayfish of 27.3 +/- 0.56 g mean weight and 10.0 +/- 0.72 cm mean length were selected for the bioassay experiments. The experiments were repeated three times in 20 liters of tap water. The temperature of water was 21 +/- 1 degrees C. The data obtained were statistically evaluated by using a computer program developed by the United States Environmental Protection Agency, based on Finney's probit analysis method and the 96-h LC50 value for crayfish was calculated to be 15.75 mu g/L. The 95% lower and upper confidence limits for the LC50 were 9.45 to 25.01 mu g/L. In addition to the acute toxicity bioassay experiments, 24-h oxidative stress parameters such as malondialdehyde (MDA) levels and ferrous oxidation assay (FOX HP[ hydrogen peroxide] equivalents) were also determined. Only MDA levels of hepatopancreas decreased at 5, 10, and 20 mu g/L of fenitrothion doses. We can conclude that fenitrothion is highly toxic to crayfish, a nontarget organism in the ecosystem, and the lipid peroxidation indicators can be easily used for monitoring environmental effects. C (C) 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25: 169-174, 2011; View this article online at wileyonlinelibrary.com. DOI 10:1002/jbt.20373