Does bisphenol A bioaccumulate on zebrafish? Determination of tissue bisphenol A level

Eser B., Tural R., Gunal A. Ç., Sepici Dincel A.

BIOMEDICAL CHROMATOGRAPHY, cilt.36, sa.3, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 36 Sayı: 3
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1002/bmc.5285
  • Dergi Adı: BIOMEDICAL CHROMATOGRAPHY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, EMBASE, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: bioaccumulation, bisphenol A, endocrine-disrupting chemicals, HPLC, zebrafish, ENDOCRINE-DISRUPTING CHEMICALS, TAIHU LAKE, WATER, EXPOSURE, SEDIMENT, RECEPTOR, HORMONES, IMPACT, BPA
  • Gazi Üniversitesi Adresli: Evet

Özet

Bisphenol A (BPA) is a high-production-volume industrial chemical mainly used in the production of polycarbonates and epoxy resins utilized in the manufacture of containers, bottles, toys, and medical devices. It has systemic effects as an endocrine disruptor even at low doses. To analyze its quantity in biological materials, sensitive and reproducible methods have to be used. Different doses and duration (90 and 900 mu g/L, 24 and 120 h, and 21 days) of BPA exposure to whole body zebrafish were analyzed after specific homogenization of tissue, and then a modified method HPLC was used. The mobile phase was acetonitrile and water using a gradient method of reversed-phase C18 column, and excitation = 227 nm/emission = 313 nm. The calibration curve for BPA using HPLC-fluorescence detection method was between a concentration range of 1 and 1000 ng/mL and linear, and r(2) = 0.999. The mean and standard error of mean values were 4.29 +/- 1.05, 2.50 +/- 0.92, and 2.53 +/- 0.68 for control; 10.43 +/- 2.61, 11.46 +/- 3.24, and 8.55 +/- 3.11 for BPA-90 mu g/L; and 17.78 +/- 4.39, 21.55 +/- 4.37, and 25.32 +/- 3.25 for BPA-900 mu g/L (24 h, 120 h, and 21 days, respectively). Although some statistical significance among dose/time was observed between two different dose-treated groups, statistical significance was not found in dose/time results within the group. However, the positive result of BPA in the control group can be explained by low-dose, chronic exposure or prevalence of endocrine-disrupting chemicals.