Bioactivities of Ethanolic Extract and its Fractions of Cistus laurifolius L. (Cistaceae) and Salvia wiedemannii Boiss. (Lamiaceae) Species.

Creative Commons License

Üstün O., Berrin-Ozcelik B., Baykal T.

Pharmacognosy magazine, cilt.12, sa.Suppl 1, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 12 Sayı: Suppl 1
  • Basım Tarihi: 2016
  • Doi Numarası: 10.4103/0973-1296.176125
  • Dergi Adı: Pharmacognosy magazine
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: Antibacterial activity, antifungal activity, antiviral activity, Cistus laurifolius, cytotoxicity, Salvia wiedemannii, TRADITIONAL MEDICINE, FOLK MEDICINE, VIRUS TYPE-1, IN-VITRO, TURKEY, ANATOLIA, MILTIORRHIZA, ANTIOXIDANT, FLAVONOIDS, PROVINCES
  • Gazi Üniversitesi Adresli: Evet

Özet

Background: Cistus laurifolius L. (Cistaceae) and Salvia wiedemannii Boiss. (Lamiaceae) have been used for treatment of some illnesses in Turkish folk medicine. In the present study, the ethanolic extract and its fractions obtained using re-extraction by hexane (Hx), chloroform (CHCl3), butanol, and remaining-water (r-H2O) of C. laurifolius were screened for their in vitro bioactivities. Materials and Methods: Activities were determined against both standard and the isolated strains of Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae, Acinetobacter baumannii, Staphylococcus aureus, Enterococcus faecalis, as well as yeasts such as Candida albicans and Candida parapsilosis by microdilution method. Also, antiviral activity of C. laurifolius and S. wiedemannii extracts were tested on herpes simplex virus-1 (HSV-1) and parainfluenza-3 (PI-3) using Madin-Darby bovine kidney and vero cell lines. Results: Tested extracts of C. laurifolius (minimum inhibitory concentration 32 mu g/mL) exerted a strong antimicrobial activity against Gram-negative bacteria of E. coli, P. mirabilis, K. pneumoniae, and A. baumannii. Conclusion: The Hx extract of C. laurifolius (cytopathogenic effect of 32-8 mu g/mL) had antiviral activity on PI-3. Also, the r-H2O, CHCl3, and ethanol extracts (16-<0.25 mu g/mL) of S. wiedemannii had significant antiviral activity on HSV-1, same as control.