Cloning and expression of a plasmid-linked pediocin determinant trait of Pediococcus acidilactici F


Osmanagaoglu O., Beyatli Y., Gunduz U.

JOURNAL OF BASIC MICROBIOLOGY, vol.40, no.1, pp.41-49, 2000 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 40 Issue: 1
  • Publication Date: 2000
  • Title of Journal : JOURNAL OF BASIC MICROBIOLOGY
  • Page Numbers: pp.41-49

Abstract

Plasmid DNA from Pediococcus acidilactici F was prepared by lysozyme-mutanolysin method and purified by cesium chloride-ethidium bromide (CsCl-EtBr) density gradient ultracentrifugation. Agarose gel electrophoresis of plasmid DNA and plasmid-curing experiments suggested that bacteriocin activity was harboured on a small plasmid of approximately 9.1 kb (kilobasepair) in Pediococcus acidilactici F. Plasmid encoding bacteriocin production in P. acidilactici F was examined for restriction enzyme cleavage patterns and its map has been constructed. An Escherichia coli strain transformed with the recombinant plasmid, pQE322, produced and, most probably, secreted pediocin F.