Quality Control of the Qualitative Real Time PCR Method for the Detection of Aspergillus DNA in Clinical Samples


Ozyegen Arslan S., Aydin M., Jabban I. I. K. , Seyer A., Fouad A. A. , Yalcin B., ...More

FLORA INFEKSIYON HASTALIKLARI VE KLINIK MIKROBIYOLOJI DERGISI, vol.23, no.3, pp.108-115, 2018 (Journal Indexed in ESCI) identifier

  • Publication Type: Article / Article
  • Volume: 23 Issue: 3
  • Publication Date: 2018
  • Doi Number: 10.5578/flora.66516
  • Title of Journal : FLORA INFEKSIYON HASTALIKLARI VE KLINIK MIKROBIYOLOJI DERGISI
  • Page Numbers: pp.108-115

Abstract

Introduction: The culture of clinical samples is positive in only 50% of the aspergillosis cases. In most neutropenic fever patients, anti-fungal treatment is generally commenced based on empirical considerations and reduces the growth rates in microbiological cultures. Polymerase chain reaction (PCR) screening for circulating DNA is not related to treatment in order for the DNA to be detected under antifungal effect. We evaluated the utility of qualitative real time PCR assay for the detection of Aspergillus DNA.