Quality Control of the Qualitative Real Time PCR Method for the Detection of Aspergillus DNA in Clinical Samples

Ozyegen Arslan, Sevgi; Aydin, Merve; Jabban, Israa; Seyer, Ayse; Fouad, Ali; Yalcin, Burce; Demir, Feyza; Biter, Gulsah; KALKANCI, AYŞE; Kustimur, Semra

doi:10.5578/flora.66516

Quality Control of the Qualitative Real Time PCR Method for the Detection of Aspergillus DNA in Clinical Samples

Ozyegen Arslan S., Aydin M., Jabban I. I. K., Seyer A., Fouad A. A., Yalcin B., ...Daha Fazla

FLORA INFEKSIYON HASTALIKLARI VE KLINIK MIKROBIYOLOJI DERGISI, cilt.23, sa.3, ss.108-115, 2018 (ESCI, TRDizin)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 23 Sayı: 3
Basım Tarihi: 2018
Doi Numarası: 10.5578/flora.66516
Dergi Adı: FLORA INFEKSIYON HASTALIKLARI VE KLINIK MIKROBIYOLOJI DERGISI
Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), TR DİZİN (ULAKBİM)
Sayfa Sayıları: ss.108-115
Gazi Üniversitesi Adresli: Evet

Özet

Introduction: The culture of clinical samples is positive in only 50% of the aspergillosis cases. In most neutropenic fever patients, anti-fungal treatment is generally commenced based on empirical considerations and reduces the growth rates in microbiological cultures. Polymerase chain reaction (PCR) screening for circulating DNA is not related to treatment in order for the DNA to be detected under antifungal effect. We evaluated the utility of qualitative real time PCR assay for the detection of Aspergillus DNA.